MSI Diagnostics
Innovation
New and fast matrix application technology
Science
Cooperation with a cutting edge scientific community
Simplification
All-in-one-kit to simplify your work
Many laboratory experiments are driven by trial and error, which can be very frustrating! We have experienced this frequently in the field of MALDI mass spectrometry imaging. You often have to do an extensive preparation with many potential sources of error before you get your analysis results. Not any more!
We are developing a complete package for a simplified and accelerated MSI preparation – including a matrix deposition device, a suitable matrix, special slides and an integrated calibration system.
Now you can focus on analyzing your data, instead of tediously acquiring it!
Now you can focus on analyzing your data, instead of tediously acquiring it!
Composition of the Preparation-Kit
Slide
Object slide with ITO or gold coating
Calibration System
Calibrant is already applied to the slide
MAPS Matrix
Novel MAPS matrix detects even small molecules
Dipping apparatus MSI-360
Fast matrix deposition by dipping
Preparation-Kit
Fast, easy and standardized. The all-in-one-preparation-kit facilitates your tissue preparation for the MALDI-MSI analysis.
Preparation-Kit: Not available yet. Still under development. Individual components will be available soon.
Slide
Are the slides suitable for MALDI-MSI?
The static charge is dissipated by the indium tin oxide (ITO) or our gold coating. In addition, the slides measure 25 x 75 mm and are transparent. All these properties make them suitable for analysis using mass spectrometry imaging.
Slides: Will be available soon!
Calibration System
What should be calibrated? Which calibration substance is suitable? How should the calibrant be applied?
These are the questions that the user no longer has to deal with in the future. The calibration system is already mounted on each slide. As soon as the tissue has been picked up with the slide, and the matrix is applied, the MSI measurement can be started. The user saves time and can concentrate on analyzing the results.
Calibration system: Under development!
MAPS
What are the properties of MAPS?
The new MAPS matrix is suitable for the visualization of small molecules (< 250 m/z) such as lactate, 2-hydroxyglutarate, and chloride anions. The chemical characteristics of MAPS offer the opportunity for an innovative matrix deposition method with our dipping apparatus.
MAPS matrix: Will be available soon!
Dipping Device
MSI-360 impresses with its simplicity.
Our matrix deposition device is immediately ready for use. There is no need for a time consuming preparation before matrix application, e.g. rinsing or changing solvents. When the matrix is placed in the device and the slide is anchored in the carrier, you only have to press a button and the dipping process will be performed automatically. The slide is ready for your MALDI-MSI measurement within approximately 5 minutes (depending on the solvent).
MSI-360: Will be available soon!
FAQs
Who is behind MSI Diagnostics?
The MSI Diagnostics team consists of three graduates of the University/ FH Bielefeld. Thanks to the open-minded professor Dr. Karsten Niehaus and the Biomedical Imaging Group of the University Bielefeld, we were able to pursue our concept and elaborate our founding idea. The idea soon turned out to be very promising. The MSI Diagnostics team received an EXIST-Gründerstipendium (Sep 2017 – Okt 2018), which is a grant from the European Union and the German Federal Ministry of Economics and Technology. In addition we were awarded a membership to the InnovationslaborOWL and won the first prize of the startklar Businessplanwettbewerb OWL (2018) and the WrapUp Event (2018). The Gründerstipendium.NRW makes it possible to facilitate the start into the world of entrepreneurs (Dec 2018 – Nov 2019).
Which tissues have already been analyzed with the matrix MAPS?
Until now, nerve tissue (rat), kidney tissue, liver tissue (mouse), brain tumor tissue (Glioblastoma multiforme), heart tissue, arterial tissue (human) as well as plant tissue (Atropa belladonna) have been successfully measured.
Which molecules can be detected with the MAPS matrix?
For a list of measured m/z-values (< 250 m/z) and correlating metabolites see the following link: Measured metabolites
What kind of calibration are you developing?
We are developing a calibration system, which includes several calibrations, such as mass calibration, quantification and lateral calibration.
For mass calibration we use phosphorus clusters, which are easily formed from red phosphorus by laser desorption ionization. Red phosphorus is ideal for calibration because it is monoisotopic, the clusters are singly charged, and a range of up to approximately m/z 3000 can be covered.
For mass calibration we use phosphorus clusters, which are easily formed from red phosphorus by laser desorption ionization. Red phosphorus is ideal for calibration because it is monoisotopic, the clusters are singly charged, and a range of up to approximately m/z 3000 can be covered.
What is required for the operation of the diving device?
You need a fume hood with a small space of 15 cm x 30 cm x 20 cm, a waste container and a power connection nearby.
How long does a matrix deposition take with the dipping apparatus?
On average the matrix application takes about 5 minutes (depending on the solvent). This time includes both the application of the matrix and the time needed to clean the MSI-360.
Feel free to contact us in case you have questions, need advice, or recommendations for the right settings of your dipping apparatus MSI-360.
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